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The buffer was twenty mM imidazole pH seven, with the addition of 1 mM DTT and 1 mM EDTA when T6P synthase was assayed. The extract was centrifuged in the cold for fifteen min at 13000 rpm in an Eppendorf table leading centrifuge and the supernatant utilised for determination of enzyme activities. T6P synthase exercise was decided by a two phase approach in a combination of fifty mM imidazole pH 7
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